HOW TO PERFORM GSEA - A tutorial on gene set enrichment analysis for RNA-seq

Video Description

In this tutorial, we explain what gene set enrichment analysis (GSEA) is and what it offers you. We show you how to run the analysis on your computer and take you through how to interpret the outputs. The tutorial also covers leading edge analysis and analysis of gene networks with Cytoscape. *** Editors note: If you are having trouble loading your gct file, you should save it as .gct.txt rather than just .txt The timestamps for the following sections are: 0:09 What is GSEA? 9:09 What software do I need? 9:55 What input files do I need to create? 14:12 What gene set files are available? 19:04 How do I use GSEA software? 35:57 What is leading edge analysis? 40:05 Visualising enriched gene sets as a network GSEA website (download free software here) https://www.gsea-msigdb.org/gsea/index.jsp GSEA User Guide https://www.gsea-msigdb.org/gsea/doc/GSEAUserGuideFrame.html The Gene Set Database https://www.gsea-msigdb.org/gsea/msigdb/index.jsp Cytoscape network analysis software https://cytoscape.org/ This tutorial on GSEA is brought to you by Dr Katherine West in the College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland. Look out for our other videos in this tutorial series that will help you get the most out of your gene expression analysis. We hope you found this video useful. Please support us by liking the video and consider subscribing for more informative content. Leave us a comment if you thought this video was helpful or if there is further information you would like to share with us and the community. Thank you. https://www.gla.ac.uk/people/katherinewest https://twitter.com/GenomicsGurus https://www.facebook.com/GenomicsGurus