Volcano plots with ggplot2 for differential gene expression | Beginner-friendly R

Biostatsquid • November 11, 2022
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Biostatsquid

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Welcome to Biostatsquid! My name is Laura, I am a bioinformatician who loves finding new ways to analyse and visualise biological data - from scRNAseq to proteomics! I started this channel as a way to share my experiences, teach but also learn from others! Coding and stats can be as (or more!) fun as lab work, and everyone can learn how to do it! In this channel you'll find explanations and hands-on tutorials for analysing and interpreting biological data. - Learn basic techniques in biostatistics, from heatmaps to pathway enrichment analysis. - Manage your data and create publication-ready visualisations by following my easy step-by-step tutorials in R and Python. - Practical explanations without complex formulas for you to understand your datasets and be able to interpret your results. What are you waiting for? Just dive in!

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In this video I will explain how to create and customise your own volcano plot using R. I will give you a step by step explanation and code to create and customise your own volcano plot in R to visualise your DEG results. Data & step-by-step code explanation: https://biostatsquid.com/volcano-plots-r-tutorial/ Bacher T cell data: https://rdrr.io/github/LTLA/scRNAseq/man/BacherTCellData.html Hope you like it! -------------------------------------------------------------------------------------------------------------------- Watched it already? If you liked this video or found it useful, please let me know! Your comments and feedback are very much appreciated😊 Like, subscribe and share with someone who might find it useful! If you have questions, don't hesitate to leave me a comment down below, I will answer as soon as I can:) -------------------------------------------------------------------------------------------------------------------- For more biostatistics tools and resources, you can visit: https://biostatsquid.com/ for more • simple and clear explanations of biostatistics methods • computational biology tools • easy step-by-step tutorials in R and Python to analyse and visualise your biological data! Or follow me on Instagram at @biostatsquid: https://www.instagram.com/biostatsquid/ Don’t forget to subscribe if you don’t want to miss another video from me! -------------------------------------------------------------------------------------------------------------------- More volcano plots resources: In this video, I explain how to create your own volcano plot in R: https://www.youtube.com/watch?v=sIRnaKo1aKE This is also a really cool tool made with RShiny which allows you to customise your own volcano plot online: https://huygens.science.uva.nl/VolcaNoseR/ And here is a nice, short explanation on how to interpret volcano plots: https://www.htgmolecular.com/blog/2022-08-25/understanding-volcano-plots

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